Following are some examples of gene sets that can be explored with GeneAnalytics:

• Differentially expressed genes identified by microarray experiment, RNA sequencing, real-time PCR or any other molecular method
• Gene sets that contain genomic variants known to be related to a specific disease or group of diseases
• Genes that encode protein targets of a specific drug
• Genes encoding proteins known to be a part of a specific molecular pathway or process

For detailed examples, view our case studies.

• Official gene symbols only!
• GeneAnalytics is designed to identify mouse and human gene symbols. The user must indicate the query species to enable the identification procedure but this indication does not impact the results.

• Currently, GeneAnalytics is recommended for the analysis of gene sets that contain 300 or fewer genes. Analyzing longer lists may yield biased results, with over-representation of entities that contain higher number of genes.  

  If you insert a gene set with more than 300 genes, you will be asked whether you want to proceed with your long set, or to trim the list to 300 genes. If you choose to trim the list, the first 300 genes will be used (duplicate genes will be removed automatically). 

   

You can insert your gene symbols by either:

1. Typing in the gene(s) symbol in the input window, one gene at a time. Use the auto-complete feature to define the correct official gene symbol.
2. Pasting in a list of genes into the input window. The pasted genes automatically undergo an identification procedure. 
3. Uploading a file containing the gene list. Only text files are accepted. The uploaded genes automatically undergo an identification procedure.

• GeneAnalytics is designed to identify mouse and human gene symbols. The user must indicate the query species to enable the identification procedure.  However, the query species does not impact the results:
  • The expression matching algorithm is performed on all the genes available in LifeMap Discovery®, regardless of their species (human, mouse, rat, chicken, pig).
  • The matching algorithm used in the diseases, pathways, GO terms and compounds sections converts all gene symbols into human gene symbols.
• Gene symbols from another species can be queried, but GeneAnalytics will identify them according to the official mouse or human gene symbols.
• Please note that changing the input species after inserting genes will automatically result in a new identification process. 

GeneAnalytics does not enable data normalization and calculation of differentially expressed genes.

However, we offer a service for identifying differentially expressed genes from microarray experiments. For more information, please contact us. 

Unidentified genes are those which were not recognized as official mouse or human gene symbols.

• Unidentified genes are genes that were not recognized as official human or mouse gene symbols.
• Note:  unidentified genes are excluded from the analysis and do not impact its results.
• To correct unidentified gene symbols perform one of the following:

1. Click the ‘edit’ icon  and start typing in your postulated gene symbol. Use the auto-complete function to identify the correct symbol.
2. Click the ‘search in GeneCards’ icon   . Then, copy the correct symbol from GeneCards® into GeneAnalytics.
3. Try to switch the selected input species from human to mouse or vice versa. Note that changing the input species after inserting gene symbols will lead to a new identification process.

• Try to maximize the number of identified genes in your query by searching for the official gene symbols for your unidentified genes (see: How can I correct my unidentified genes?).
• In the results page, examine the ‘notes’ in the ‘analyzed genes’ panel. Consider removing abundant and housekeeping genes from your query, particularly if you are interested in expression analysis, since including such genes can result in nonspecific results).